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Indeed, any mutation in this region causes a loss ofp53 transcriptional activity [ 35], since it leads to either an alteration in the residueswhich determine a sequence-speci?c binding to the DNA (class I mutants, e.g.mutants at codons 248 or 273), or a change of p53 folding, abolishing completely thebinding to the DNA and changing the capability to interact with other proteins (classII mutants, e.g. For the patient to generate inspiratory air-?ow in the next breath, the patient must generatea negative pressure equal in magnitude to theopposing elastic recoil pressures. Kobet E et al (2000) MDM2 inhibits p300-mediated p53 acetylation and activation by forminga ternary complex with the two proteins.

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